Interactions between Fe-doped imogolites nanotubes and the soil bacteria P. Brassecacearum. - CNRS - Centre national de la recherche scientifique Access content directly
Conference Papers Year : 2016

Interactions between Fe-doped imogolites nanotubes and the soil bacteria P. Brassecacearum.

A. Avellan,
  • Function : Author
C. Levard
C. Santaella
  • Function : Author
W. Achouak
  • Function : Author
J. Rose
  • Function : Author


Biogenic silica resulting from the precipitation of dissolved Si through biological processes in plants, often contains small amounts of occluded organic carbon. These phases, called phytoliths, have a long persistence in soils, making them tracers of past conditions. In this context, the knowledge of the carbon speciation associated with phytoliths bears significant importance in examining the carbon dynamics in soils. With carbon concentrations as low as the 0.1% range, examining the nature of organic carbon remains very challenging, and available tools (e.g. pyrolysis) are often prone to serious artifacts. Recent improvements of microwave sources enabled the application of the Dynamic Nuclear Polarization (DNP) technique to NMR, thereby establishing a new non-destructive tool for the qualitative and quantitative determination of the carbon speciation. Applied to the analysis of phytoliths, this method showed the presence of carbons from different sources within the sample: About 20% of the signal correspond to carbohydrates, and are assigned to photosynthetic carbon; the marked alkyl, N-alkyl and carbonyl signals indicate a significant proportion of proteins. This is consistent with the hypothesis that parts of the carbon associated with the phytoliths is imported into the host plant via uptake from the soil. Finally, lignins, glomalin-like and/or humic-like compounds are minor species associated with biogenic silica. This speciation was obtained overnight with a DNP-NMR setup with an excellent sensitivity (few tens of weight ppm); the same spectrum on a standard NMR spectrometer would have required at least 250 days of data acquisition. The considerable gain in sensitivity associated with the use of DNP now makes NMR a relevant technique for the analysis of environmental samples. Session B081 The fate of carbon in plants and terrestrial ecosystems-from respiration to allocation.
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hal-03547683 , version 1 (09-02-2022)


  • HAL Id : hal-03547683 , version 1


A. Avellan,, A. Masion, C. Levard, C. Santaella, W. Achouak, et al.. Interactions between Fe-doped imogolites nanotubes and the soil bacteria P. Brassecacearum.. 11th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials, ICEENN, Aug 2016, Golden, CO, United States. ⟨hal-03547683⟩
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