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Article Dans Une Revue Nature Structural and Molecular Biology Année : 2017

Histone propionylation is a mark of active chromatin

Adam Kebede
  • Fonction : Auteur
Anna Nieborak
  • Fonction : Auteur
Lara Shahidian
  • Fonction : Auteur
Florian Richter
  • Fonction : Auteur
Diana Gómez
  • Fonction : Auteur
Marijke Baltissen
  • Fonction : Auteur
Aaron Taudt
  • Fonction : Auteur
Raphael Margueron
  • Fonction : Auteur
Maria Colomé-Tatché
  • Fonction : Auteur
Michiel Vermeulen
  • Fonction : Auteur
Gerhard Mittler
  • Fonction : Auteur

Résumé

Histones are highly covalently modified, but the functions of many of these modifications remain unknown. In particular, it is unclear how histone marks are coupled to cellular metabolism and how this coupling affects chromatin architecture. We identified histone H3 Lys14 (H3K14) as a site of propionylation and butyrylation in vivo and carried out the first systematic characterization of histone propionylation. We found that H3K14pr and H3K14bu are deposited by histone acetyltransferases, are preferentially enriched at promoters of active genes and are recognized by acylation-state-specific reader proteins. In agreement with these findings, propionyl-CoA was able to stimulate transcription in an in vitro transcription system. Notably, genome-wide H3 acylation profiles were redefined following changes to the metabolic state, and deletion of the metabolic enzyme propionyl-CoA carboxylase altered global histone propionylation levels. We propose that histone propionylation, acetylation and butyrylation may act in combination to promote high transcriptional output and to couple cellular metabolism with chromatin structure and function.

Dates et versions

hal-03677811 , version 1 (24-05-2022)

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Citer

Adam Kebede, Anna Nieborak, Lara Shahidian, Stéphanie Le Gras, Florian Richter, et al.. Histone propionylation is a mark of active chromatin. Nature Structural and Molecular Biology, 2017, ⟨10.1038/nsmb.3490⟩. ⟨hal-03677811⟩
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