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Article Dans Une Revue Science Année : 2021

NIN-Like Protein Transcription factors regulate leghemoglobin genes in legume nodules

Suyu Jiang
Jinpeng Gao
Jiangqi Wen
Kirankumar Mysore
Ping Xu
  • Fonction : Auteur
Carmen Sanchez-Canizares
Yiting Ruan
Qiujiu Li
Meijun Zhu
  • Fonction : Auteur
Fuyu Li
Ertao Wang
Phillip S Poole
Jeremy D Murray
  • Fonction : Auteur
  • PersonId : 1089073

Résumé

Leghemoglobins enable endosymbiotic N2-fixation in legume nodules by channelling O2 for bacterial respiration while maintaining a microoxic environment to protect O2-sensitive nitrogenase. We show that the NIN-like Protein (NLP) transcription factors NLP2 and Nodule Inception (NIN) directly activate expression of leghemoglobins through a promoter motif resembling a 'double' version of the Nitrate Responsive Elements (NREs) targeted by other NLPs that has conserved orientation and position across legumes. CRISPR-KO of the NRE-like element resulted in strongly decreased expression of the associated leghemoglobin. Our findings indicate that the origins of the NLP-leghemoglobin module for O2-buffering in nodules can be traced to an ancient pairing of NLPs with non-symbiotic hemoglobins that function in hypoxia.
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Dates et versions

hal-03728305 , version 1 (20-07-2022)

Identifiants

Citer

Suyu Jiang, Marie-Françoise Jardinaud, Jinpeng Gao, Yann Pecrix, Jiangqi Wen, et al.. NIN-Like Protein Transcription factors regulate leghemoglobin genes in legume nodules. Science, 2021, 374 (6567), pp.625-628. ⟨10.1126/science.abg5945⟩. ⟨hal-03728305⟩
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