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Article Dans Une Revue EMBO Journal Année : 2010

A long nuclear-retained non-coding RNA regulates synaptogenesis by modulating gene expression

Résumé

A growing number of long nuclear-retained non-coding RNAs (ncRNAs) have recently been described. However, few functions have been elucidated for these ncRNAs. Here, we have characterized the function of one such ncRNA, identified as metastasis-associated lung adenocarcinoma transcript 1 (Malat1). Malat1 RNA is expressed in numerous tissues and is highly abundant in neurons. It is enriched in nuclear speckles only when RNA polymerase II-dependent transcription is active. Knock-down studies revealed that Malat1 modulates the recruitment of SR family pre-mRNA-splicing factors to the transcription site of a transgene array. DNA microarray analysis in Malat1-depleted neuroblastoma cells indicates that Malat1 controls the expression of genes involved not only in nuclear processes, but also in synapse function. In cultured hippocampal neurons, knock-down of Malat1 decreases synaptic density, whereas its over-expression results in a cell-autonomous increase in synaptic density. Our results suggest that Malat1 regulates synapse formation by modulating the expression of genes involved in synapse formation and/or maintenance.

Dates et versions

hal-03790142 , version 1 (28-09-2022)

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Citer

Delphine Bernard, Kannanganattu Prasanth, Vidisha Tripathi, Sabrina Colasse, Tetsuya Nakamura, et al.. A long nuclear-retained non-coding RNA regulates synaptogenesis by modulating gene expression. EMBO Journal, 2010, 29 (18), pp.3082-3093. ⟨10.1038/emboj.2010.199⟩. ⟨hal-03790142⟩
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