Context-dependent transcriptional remodeling of TADs during differentiation - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2022

Context-dependent transcriptional remodeling of TADs during differentiation

Résumé

Metazoan chromosomes are organized into discrete domains (TADs), believed to contribute to the regulation of transcriptional programs. Despite extensive correlation between TAD organization and gene activity, a direct mechanistic link is unclear, with perturbation studies often showing little effect. To follow TAD dynamics during development, we used Capture Hi-C to interrogate the TADs around key differentially expressed genes during mouse thymocyte maturation, uncovering specific remodeling events. Notably, one TAD boundary was broadened to accommodate RNA polymerase elongation past the border, and sub-domains were formed around some activated genes without changes in CTCF binding. The ectopic induction of one gene was sufficient to recapitulate microdomain formation in embryonic stem cells, providing strong evidence that transcription can directly remodel chromatin .
Fichier principal
Vignette du fichier
Chahar_bioRxiv_2022.pdf (8.1 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03797986 , version 1 (05-10-2022)

Identifiants

Citer

Sanjay Chahar, Yousra Ben Zouari, Hossein Salari, Anne Molitor, Dominique Kobi, et al.. Context-dependent transcriptional remodeling of TADs during differentiation. 2022. ⟨hal-03797986⟩
94 Consultations
54 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More