Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa - CNRS - Centre national de la recherche scientifique Access content directly
Journal Articles Frontiers in Plant Science Year : 2021

Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa

Cintia Jozefkowicz
  • Function : Author
Cristina Gómez
  • Function : Author
Ariel Odorizzi
  • Function : Author
Anelia Iantcheva
  • Function : Author
Nicolás Ayub
  • Function : Author
Gabriela Soto
  • Function : Author

Abstract

Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves ( mfl ) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops.

Dates and versions

hal-03860573 , version 1 (18-11-2022)

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Cintia Jozefkowicz, Cristina Gómez, Ariel Odorizzi, Anelia Iantcheva, Pascal Ratet, et al.. Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa. Frontiers in Plant Science, 2021, 12, ⟨10.3389/fpls.2021.805032⟩. ⟨hal-03860573⟩
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