On the unknown proteins of eukaryotic proteomes - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2023

On the unknown proteins of eukaryotic proteomes

Résumé

In order to study unknown proteins on a large scale, a reference system has been set up for the three major eukaryotic lineages, built with 36 proteomes as taxonomically diverse as possible. Proteins from 362 eukaryotic proteomes with no known homologue in this set were then analyzed, focusing noteworthy on singletons, that is, on unknown proteins with no known homologue in their own proteome. Consistently, according to Uniprot, for a given species, no more than 12% of the singletons thus found are known at the protein level. Also, since they rely on the information found in the alignment of homologous sequences, predictions of AlphaFold2 for their tridimensional structure are usually poor. In the case of metazoan species, the number of singletons seems to increase as a function of the evolutionary distance from the reference system. Interestingly, no such trend is found in the cases of viridiplantae and fungi, as if the timescale on which singletons are added to proteomes were different in metazoa and in other eukaryotic kingdoms. In order to confirm this phenomenon, further studies of proteomes closer to those of the reference system are however needed.
Fichier principal
Vignette du fichier
singletons_eukaryotes_yhs2022_tohal.pdf (1.52 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03863835 , version 1 (02-04-2024)

Identifiants

Citer

Yves-Henri Sanejouand. On the unknown proteins of eukaryotic proteomes. 2024. ⟨hal-03863835⟩
9 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More