IN VITRO MICROFLUIDIC DESIGN TO STUDY MITOCHONDRIA-MICROTUBULES INTERACTIONS AFTER AN AXONAL TRAUMATIC INJURY - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Poster De Conférence Année : 2022

IN VITRO MICROFLUIDIC DESIGN TO STUDY MITOCHONDRIA-MICROTUBULES INTERACTIONS AFTER AN AXONAL TRAUMATIC INJURY

Résumé

A lesion in the nervous system induces cellular and molecular mechanisms leading to irreversible dysfunctions. Post-injured environment is non permissive for axonal regeneration and CNS neurons also exhibit a reduced intrinsic growth capacity. Nevertheless, neuronal plasticity is still possible: injured axons can degenerate or regenerate, but mechanisms governing this response remains elusive. My aim is to understand the relation between microtubules and mitochondria on axon fate after a traumatic injury, in particular by focusing on the corticospinal tract connecting to the spinal cord neurons. Corticospinal neurons are known to show poor regeneration capacities after a spinal cord injury. Axonal degeneration is associated with a fast decrease of NAD+ leading to energy drop, cytoskeletal disorganization and finally axonal degradation. Mitochondria repositioning in the axon can stimulate regeneration and branching by rescuing energy deficits. In addition, microtubules (MT) to which converge extracellular cues for intracellular trafficking are also involved in mitochondrial transport. Thereby, mitochondria and MT are essential in determining the axon fate. I am developping an in vitro set-up with microfluidics oriented networks of cortico-spinal neurons on which a physical injruy can be apply. This system will allow to study the influence of post-translationnal MT modifications on mitochondria dynamic during axonal regeneration or degeneration.
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Dates et versions

hal-03946575 , version 1 (19-01-2023)

Identifiants

  • HAL Id : hal-03946575 , version 1

Citer

Theo Andriot, Chloé Dupuis, Teng Pan, Jean-Michel Peyrin, Fatiha Nothias, et al.. IN VITRO MICROFLUIDIC DESIGN TO STUDY MITOCHONDRIA-MICROTUBULES INTERACTIONS AFTER AN AXONAL TRAUMATIC INJURY. FENS, Jul 2022, Paris, France. ⟨hal-03946575⟩
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