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Article Dans Une Revue (Data Paper) Nature Microbiology Année : 2017

A myovirus encoding both photosystem I and II proteins enhances cyclic electron flow in infected Prochlorococcus cells

Svetlana Fridman
  • Fonction : Auteur
José Flores-Uribe
  • Fonction : Auteur
Shirley Larom
  • Fonction : Auteur
Onit Alalouf
  • Fonction : Auteur
Oded Liran
  • Fonction : Auteur
Iftach Yacoby
  • Fonction : Auteur
Faris Salama
  • Fonction : Auteur
Tamar Ziv
  • Fonction : Auteur
Itai Sharon
  • Fonction : Auteur
Francisco M Cornejo-Castillo
  • Fonction : Auteur
Alon Philosof
  • Fonction : Auteur
Christopher L Dupont
  • Fonction : Auteur
Pablo Sánchez
  • Fonction : Auteur
Silvia G Acinas
  • Fonction : Auteur
Forest L Rohwer
  • Fonction : Auteur
Debbie Lindell
  • Fonction : Auteur
  • PersonId : 1231761
Oded Béjà
  • Fonction : Auteur

Résumé

Cyanobacteria are important contributors to primary production in the open oceans. Over the past decade, various photosynthesis-related genes have been found in viruses that infect cyanobacteria (cyanophages). Although photosystem II (PSII) genes are common in both cultured cyanophages and environmental samples 1-4 , viral photosystem I (vPSI) genes have so far only been detected in environmental samples 5,6 . Here, we have used a targeted strategy to isolate a cyanophage from the tropical Pacific Ocean that carries a PSI gene cassette with seven distinct PSI genes (psaJF, C, A, B, K, E, D) as well as two PSII genes (psbA, D). This cyanophage, P-TIM68, belongs to the T4-like myoviruses, has a prolate capsid, a long contractile tail and infects Prochlorococcus sp. strain MIT9515. Phage photosynthesis genes from both photosystems are expressed during infection, and the resultant proteins are incorporated into membranes of the infected host. Moreover, photosynthetic capacity in the cell is maintained throughout the infection cycle with enhancement of cyclic electron flow around PSI. Analysis of metagenomic data from the Tara Oceans expedition 7 shows that phages carrying PSI gene cassettes are abundant in the tropical Pacific Ocean, composing up to 28% of T4-like cyanomyophages. They are also present in the tropical Indian and Atlantic Oceans. P-TIM68 populations, specifically, compose on average 22% of the PSI-gene-cassette carrying phages. Our results suggest that cyanophages carrying PSI and PSII genes are likely to maintain and even manipulate photosynthesis during infection of their Prochlorococcus hosts in the tropical oceans.
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Dates et versions

hal-04006860 , version 1 (27-02-2023)

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Svetlana Fridman, José Flores-Uribe, Shirley Larom, Onit Alalouf, Oded Liran, et al.. A myovirus encoding both photosystem I and II proteins enhances cyclic electron flow in infected Prochlorococcus cells. Nature Microbiology, 2017, 2 (10), pp.1350-1357. ⟨10.1038/s41564-017-0002-9⟩. ⟨hal-04006860⟩
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