Characterization of Tunisian Bacillus thuringiensis strains with abundance of kurstaki subspecies harbouring insecticidal activities against the lepidopteran insect Ephestia kuehniella - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Current Microbiology Année : 2010

Characterization of Tunisian Bacillus thuringiensis strains with abundance of kurstaki subspecies harbouring insecticidal activities against the lepidopteran insect Ephestia kuehniella

Imen Saadaoui
  • Fonction : Auteur
Roda Al-Thani
  • Fonction : Auteur
Fatma Al-Saadi
  • Fonction : Auteur
Najeh Belguith-Ben Hassan
  • Fonction : Auteur
Lobna Abdelkefi-Mesrati
  • Fonction : Auteur
Souad Rouis
  • Fonction : Auteur
Samir Jaoua
  • Fonction : Auteur

Résumé

The study of 257 crystal-producing Bacillus thuringiensis isolates from bioinsecticide free soil samples collected from different sites in Tunisia, was performed by PCR amplification, using six primer pairs specific for cry1, cry2, cry3, cry4, and vip3A genes, by the investigation of strain plasmid pattern, crystal morphology and delta-endotoxin content and by the assessment of insecticidal activities against the lepidopteran insect Ephestia kuehniella. Based on plasmid pattern study, 11 representative strains of the different classes were subjected to morphological and molecular analyses. The comparison of the PFGE fingerprints confirmed the heterogeneity of these strains. B. thuringiensis kurstaki strains, harbouring at the same time the genes cry1A, cry2, cry1Ia, and vip3A, were the most abundant (65.4%). 33.34% of the new isolates showed particular delta-endotoxin profiles but no PCR products with the used primer sets. B. thuringiensis israelensis was shown to be also very rare among the Tunisian B. thuringiensis isolates diversity. These findings could have considerable impacts for the set up of new pest control biological agents.

Dates et versions

hal-04014351 , version 1 (03-03-2023)

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Citer

Imen Saadaoui, Roda Al-Thani, Fatma Al-Saadi, Najeh Belguith-Ben Hassan, Lobna Abdelkefi-Mesrati, et al.. Characterization of Tunisian Bacillus thuringiensis strains with abundance of kurstaki subspecies harbouring insecticidal activities against the lepidopteran insect Ephestia kuehniella. Current Microbiology, 2010, 61 (6), pp.541-548. ⟨10.1007/s00284-010-9650-1⟩. ⟨hal-04014351⟩
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