Construction and characterisation of a knock-down RNA interference line of OsNRPD1 in rice (Oryza sativa ssp japonica cv Nipponbare) - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Philosophical Transactions of the Royal Society B: Biological Sciences Année : 2020

Construction and characterisation of a knock-down RNA interference line of OsNRPD1 in rice (Oryza sativa ssp japonica cv Nipponbare)

Sandra Mathioni
Pao-Yang Chen
Blake Meyers

Résumé

In plants, RNA-directed DNA Methylation (RdDM) is a mechanism of silencing that relies on the production of 24-nt siRNAs by RNA POLYMERASE IV (Pol IV) to trigger methylation and thus inactivation of transposable elements (TEs). We present the construction and characterisation of osnrpd1, a knock-down RNA interference line of OsNRPD1 gene that encodes the largest subunit of Pol IV in rice (Oryza sativa ssp japonica cv Nipponbare). We show that osnrpd1 displays a lower accumulation of OsNRPD1 transcripts, associated to an overall reduction of 24-nt siRNAs and DNA methylation level in all three contexts, CG, CHG and CHH. We uncovered new insertions of known active TEs, the LTR retrotransposons Tos17 and Lullaby and the LINE-type retrotransposon Karma. However, we did not observe any clear developmental phenotype, contrary to what was expected for a mutant severily affected in RdDM. In addition, despite the presence of many putatively functional TEs in the rice genome, we found no evidence of in planta global reactivation of transposition. This knock-down of OsNRPD1 likely led to a weakly affected line, with no effect on development and a limited effect on transposition. We discuss the possibility that a knockout mutation of OsNRPD1 would cause sterility in rice.
Fichier principal
Vignette du fichier
osnrpd1_hal.pdf (1.47 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04274817 , version 1 (08-11-2023)

Identifiants

Citer

Emilie Debladis, Tzuu-Fen Lee, Yan-Jiun Huang, Jui-Hsien Lu, Sandra Mathioni, et al.. Construction and characterisation of a knock-down RNA interference line of OsNRPD1 in rice (Oryza sativa ssp japonica cv Nipponbare). Philosophical Transactions of the Royal Society B: Biological Sciences, 2020, 375 (1795), pp.20190338. ⟨10.1098/rstb.2019.0338⟩. ⟨hal-04274817⟩
29 Consultations
5 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More