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Article Dans Une Revue Genome Biology Année : 2013

VlincRNAs controlled by retroviral elements are a hallmark of pluripotency and cancer

Georges St Laurent
  • Fonction : Auteur
Dmitry Shtokalo
  • Fonction : Auteur
Biao Dong
  • Fonction : Auteur
Michael R Tackett
  • Fonction : Auteur
Xiaoxuan Fan
  • Fonction : Auteur
Sandra Lazorthes
  • Fonction : Auteur
Nianli Sang
  • Fonction : Auteur
Timothy J Triche
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Timothy A Mccaffrey
  • Fonction : Auteur
Weidong Xiao
  • Fonction : Auteur
  • PersonId : 1310756
Philipp Kapranov
  • Fonction : Auteur
  • PersonId : 1310757

Résumé

Background: The function of the non-coding portion of the human genome remains one of the most important questions of our time. Its vast complexity is exemplified by the recent identification of an unusual and notable component of the transcriptome-very long intergenic non-coding RNAs, termed vlincRNAs. Results: Here we identify 2,147 vlincRNAs covering 10 percent of our genome. We show they are present not only in cancerous cells, but also in primary cells and normal human tissues, and are controlled by canonical promoters. Furthermore, vlincRNA promoters frequently originate from within endogenous retroviral sequences. Strikingly, the number of vlincRNAs expressed from endogenous retroviral promoters strongly correlates with pluripotency or the degree of malignant transformation. These results suggest a previously unknown connection between the pluripotent state and cancer via retroviral repeat-driven expression of vlincRNAs. Finally, we show that vlincRNAs can be syntenically conserved in humans and mouse and their depletion using RNAi can cause apoptosis in cancerous cells. Conclusions: These intriguing observations suggest that vlincRNAs could create a framework that combines many existing short ESTs and lincRNAs into a landscape of very long transcripts functioning in the regulation of gene expression in the nucleus. Certain types of vlincRNAs participate at specific stages of normal development and, based on analysis of a limited set of cancerous and primary cell lines, they appear to be co-opted by cancerassociated transcriptional programs. This provides additional understanding of transcriptome regulation during the malignant state, and could lead to additional targets and options for its reversal.
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Dates et versions

hal-04292255 , version 1 (17-11-2023)

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Georges St Laurent, Dmitry Shtokalo, Biao Dong, Michael R Tackett, Xiaoxuan Fan, et al.. VlincRNAs controlled by retroviral elements are a hallmark of pluripotency and cancer. Genome Biology, 2013, 14 (7), pp.R73. ⟨10.1186/gb-2013-14-7-r73⟩. ⟨hal-04292255⟩
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