Spectral decomposition unlocks ascidian morphogenesis - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2023

Spectral decomposition unlocks ascidian morphogenesis

Joel Dokmegang
Emmanuel Faure
Ed Munro
  • Fonction : Auteur
Madhav Mani

Résumé

Describing morphogenesis generally consists in aggregating the multiple high resolution spatiotemporal processes involved into repeatable low dimensional morphological processes consistent across individuals of the same species or group. In order to achieve this goal, biologists often have to submit movies issued from live imaging of developing embryos either to a qualitative analysis or to basic statistical analysis. These approaches, however, present noticeable drawbacks, as they can be time consuming, hence unfit for scale, and often lack standardisation and a firm foundation. In this work, we leverage the power of a continuum mechanics approach and flexibility of spectral decompositions to propose a standardised framework for automatic detection and timing of morphological processes. First, we quantify whole-embryo scale shape changes in developing ascidian embryos by statistically estimating the strain-rate tensor field of its time-evolving surface without the requirement of cellular segmentation and tracking. We then apply to this data spectral decomposition in space using spherical harmonics and and in time using wavelets transforms. These transformations result in the identification of the principal dynamical modes of ascidian embryogenesis and the automatic unveiling of its blueprint in the form of spectograms that tell the story of development in ascidian embryos.
Fichier principal
Vignette du fichier
2023.08.22.554368v1.full.pdf (3.95 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Licence : CC BY - Paternité

Dates et versions

hal-04310643 , version 1 (27-11-2023)

Identifiants

Citer

Joel Dokmegang, Emmanuel Faure, Patrick Lemaire, Ed Munro, Madhav Mani. Spectral decomposition unlocks ascidian morphogenesis. 2023. ⟨hal-04310643⟩
25 Consultations
6 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More