The neddylation pathway regulates the localization of meiotic CO, the pairing of homologous chromosomes and the level of DNA methylation in Arabidopsis thaliana - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Communication Dans Un Congrès Année : 2018

The neddylation pathway regulates the localization of meiotic CO, the pairing of homologous chromosomes and the level of DNA methylation in Arabidopsis thaliana

Aurélie Hurel
Marina Tagliaro-Jahns
Aurélie Chambon
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Mathilde Grelon
Nicolas Bouché
Christine Mézard

Résumé

Meiotic crossovers (COs) are important for reshuffling genetic information between homologous chromosomes and, are essential for their correct segregation. In all species studied, CO distribution along chromosomes is not homogeneous. The origin of such heterogeneity is still poorly understood but several lines of evidence point towards chromatin structure and chromosome dynamics issues (Mézard et al., 2015). We previously demonstrated that the neddylation/rubylation pathway of protein modification was a key regulator of the localization of meiotic COs in A. thaliana (Tagliaro-Jahns et al., 2014). In the axr1-/- mutant (the gene encoding the E1 enzyme of the neddylation complex), COs are mislocalized on chromosomes but their number is unaffected. We could show that, in axr1, COs were massively redistributed towards subtelomeric ends and that large central regions of the chromosomes were recombination free. This redistribution of COs led us to investigate chromosome dynamics in wild- type and axr1 meiocytes. We observed that he telomere bouquet in axr1 meiocytes was not altered. However, the pairing of chromosomes was altered in axr1-/-. We performed FISH on 3D nuclei with probes labelling either central regions of the chromosomes regions that almost do not recombine in the mutant or probes that label highly recombinogenic subtelomeric regions in axr1-/- compared to wild- type. We observed that pairing of central regions was rarely detected in axr1-/- and it was also affected in subtelomeric regions but to a lesser degree. We investigated the methylation status of DNA in the axr1 mutant. In somatic cells, DNA was dramatically hypermethylated compared to wild-type in all contexts (CG, CHG, CHH). This hypermethylation was also detected in meiotic cells by immunocytology. However, no clear correlation could be obtained between the methylation status of the DNA and the CO localization
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Dates et versions

hal-04500853 , version 1 (12-03-2024)

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  • HAL Id : hal-04500853 , version 1

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Nicolas Christophorou, Aurélie Hurel, Marina Tagliaro-Jahns, Aurélie Chambon, Daniel Vezon, et al.. The neddylation pathway regulates the localization of meiotic CO, the pairing of homologous chromosomes and the level of DNA methylation in Arabidopsis thaliana. Meiosis Gordon conference, Monica Colaiacovo, Jun 2018, New London (New Hampshire), United States. ⟨hal-04500853⟩
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