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Article Dans Une Revue (Data Paper) Journal of Cell Science Année : 2024

Reduction of cortical pulling at mitotic entry facilitates aster centration

Anne Rosfelter
Ghislain de Labbey
  • Fonction : Auteur
Janet Chenevert
  • Fonction : Auteur
Sebastien Schaub
  • Fonction : Auteur
Zoltan Machaty
  • Fonction : Auteur
Lydia Besnardeau
  • Fonction : Auteur
Daniel Gonzalez Suarez
  • Fonction : Auteur
Céline Hebras
  • Fonction : Auteur
Hervé Turlier
David Burgess
Alex Mcdougall

Résumé

ABSTRACT Equal cell division relies upon astral microtubule-based centering mechanisms, yet how the interplay between mitotic entry, cortical force generation and long astral microtubules leads to symmetric cell division is not resolved. We report that a cortically located sperm aster displaying long astral microtubules that penetrate the whole zygote does not undergo centration until mitotic entry. At mitotic entry, we find that microtubule-based cortical pulling is lost. Quantitative measurements of cortical pulling and cytoplasmic pulling together with physical simulations suggested that a wavelike loss of cortical pulling at mitotic entry leads to aster centration based on cytoplasmic pulling. Cortical actin is lost from the cortex at mitotic entry coincident with a fall in cortical tension from ∼300pN/µm to ∼100pN/µm. Following the loss of cortical force generators at mitotic entry, long microtubule-based cytoplasmic pulling is sufficient to displace the aster towards the cell center. These data reveal how mitotic aster centration is coordinated with mitotic entry in chordate zygotes.

Dates et versions

hal-04556169 , version 1 (23-04-2024)

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Citer

Anne Rosfelter, Ghislain de Labbey, Janet Chenevert, Rémi Dumollard, Sebastien Schaub, et al.. Reduction of cortical pulling at mitotic entry facilitates aster centration. Journal of Cell Science, 2024, 137 (7), ⟨10.1242/jcs.262037⟩. ⟨hal-04556169⟩
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