Knockdown of human AMPK using the CRISPR-Cas9 genome-editing system - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Chapitre D'ouvrage Année : 2018

Knockdown of human AMPK using the CRISPR-Cas9 genome-editing system

Résumé

AMP activated protein kinase (AMPK) is a critical energy sensor, regulating signaling networks involved in pathology including metabolic diseases and cancer. This increasingly recognized role of AMPK has prompted tremendous research efforts to develop new pharmacological AMPK activators. To precisely study the role of AMPK, and the specificity and activity of AMPK activators in cellular models, genetic AMPK inactivating tools are required. We report here methods for genetic inactivation of AMPK 1/ 2 catalytic subunits in human cell lines by the CRISPR/Cas9 technology, a recent breakthrough technique for genome editing.

Domaines

Autre [q-bio.OT]
Fichier principal
Vignette du fichier
chapter11.pdf (255.8 Ko) Télécharger le fichier
Figures Chapter11.pdf (196.38 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

inserm-02349913 , version 1 (05-11-2019)

Identifiants

Citer

Adrien Grenier, Pierre Sujobert, Séverine Olivier, Hélène Guermouche, Johanna Mondesir, et al.. Knockdown of human AMPK using the CRISPR-Cas9 genome-editing system. Methods Mol Biol, 1732, pp.171-194, 2018, ⟨10.1007/978-1-4939-7598-3_11⟩. ⟨inserm-02349913⟩
321 Consultations
339 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More