Fast-Folding Kinetics Using Nanosecond Laser-Induced Temperature-Jump Methods - CNRS - Centre national de la recherche scientifique Access content directly
Book Sections Year : 2022

Fast-Folding Kinetics Using Nanosecond Laser-Induced Temperature-Jump Methods

Abstract

The development of ultrafast kinetic methods is one of the factors that allowed the research on protein folding to flourish over the last twenty years. The introduction of new optical triggering techniques enabled to experimentally investigate the protein dynamics at the nanosecond to millisecond timescale, allowing researchers to test theoretical predictions and providing experimental benchmarks for computer simulations. In this work the details of how to perform kinetic experiments by the laserinduced temperature jump technique, using the two most commonly used probing techniques (namely infrared absorption and fluorescence spectroscopy) are given, with a strong emphasis on the practical details.
Fichier principal
Vignette du fichier
T-jump method_manuscript_final.pdf (560.95 Ko) Télécharger le fichier
Figures.pdf (564.77 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)

Dates and versions

pasteur-04121260 , version 1 (13-06-2023)

Identifiers

Cite

Michele Cerminara. Fast-Folding Kinetics Using Nanosecond Laser-Induced Temperature-Jump Methods. Protein Folding, 2376, Springer US, pp.117-134, 2022, Methods in Molecular Biology, 978-1-0716-1715-1. ⟨10.1007/978-1-0716-1716-8_6⟩. ⟨pasteur-04121260⟩
17 View
40 Download

Altmetric

Share

Gmail Facebook X LinkedIn More