The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration - Architecture et Fonction des Macromolécules Biologiques Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2024

The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration

Ketty Tamburrini
Sayo Kodama
Sacha Grisel
Mireille Haon
  • Function : Author
Takumi Nishiuchi
  • Function : Author
Bastien Bissaro
Yasuyuki Kubo
Jean-Guy Berrin

Abstract

Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that oxidatively degrade various polysaccharides, such as cellulose. Despite extensive research on this class of enzymes, the role played by their C-terminal regions predicted to be intrinsically disordered (dCTR) has been overlooked. Here, we investigated the function of the dCTR of an LPMO, called Co AA9A, up-regulated during plant infection by Colletotrichum orbiculare , the causative agent of anthracnose. After recombinant production of the full-length protein, we found that the dCTR mediates Co AA9A dimerization in vitro, via a disulfide bridge, a hitherto-never-reported property that positively affects both binding and activity on cellulose. Using SAXS experiments, we show that the homodimer is in an extended conformation. In vivo, we demonstrate that gene deletion impairs formation of the infection-specialized cell called appressorium and delays penetration of the plant. Using immunochemistry, we show that the protein is a dimer not only in vitro but also in vivo when secreted by the appressorium. As these peculiar LPMOs are also found in other plant pathogens, our findings open up broad avenues for crop protection.
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hal-04520728 , version 1 (25-03-2024)

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Ketty Tamburrini, Sayo Kodama, Sacha Grisel, Mireille Haon, Takumi Nishiuchi, et al.. The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proceedings of the National Academy of Sciences of the United States of America, 2024, 121 (13), ⟨10.1073/pnas.2319998121⟩. ⟨hal-04520728⟩
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