Protocol to generate, purify, and analyze antibody-oligonucleotide conjugates from off-the-shelf antibodies - Département Recherches Subatomiques
Journal Articles STAR Protocols Year : 2024

Protocol to generate, purify, and analyze antibody-oligonucleotide conjugates from off-the-shelf antibodies

Abstract

Protocol to generate, purify, and analyze antibody-oligonucleotide conjugates from offthe-shelf antibodies Antibody-oligonucleotide conjugates (AOCs) are a fast-expanding modality for targeted delivery of therapeutic oligonucleotides to tissues. Here, we present a protocol to generate, purify, and analyze AOCs from off-the-shelf antibodies. We describe steps to conjugate single/doublestranded oligonucleotides bearing amine handles to linkers and, then, to antibodies using wellestablished chemistry. In addition, we provide details regarding the purification techniques and analytical methods suitable for AOC. This protocol can be applied for several purposes where AOC is a modality of interest.

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Dates and versions

hal-04728209 , version 1 (09-10-2024)

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Tony Rady, Victor Lehot, Julien Most, Stephane Erb, Sarah Cianférani, et al.. Protocol to generate, purify, and analyze antibody-oligonucleotide conjugates from off-the-shelf antibodies. STAR Protocols, 2024, 5 (4), pp.103329. ⟨10.1016/j.xpro.2024.103329⟩. ⟨hal-04728209⟩
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