A dansyl-derivatized phytic acid analogue as a fluorescent substrate for phytases: experimental and computational approach - Institut de Chimie Moléculaire de Reims - UMR 7312 Accéder directement au contenu
Article Dans Une Revue Bioorganic Chemistry Année : 2021

A dansyl-derivatized phytic acid analogue as a fluorescent substrate for phytases: experimental and computational approach

Résumé

A new myo-inositol pentakisphosphate was synthesized, which featured a dansyl group at position C-5. The fluorescent tag was removed from the inositol by a 6-atom spacer to prevent detrimental steric interactions in the catalytic site of phytases. The PEG linker was used in order to enhance hydrophilicity and biocompatibility of the new artificial substrate. Computational studies showed a favorable positioning in the catalytic site of phytases. Enzymatic assays demonstrated that the tethered myo-inositol was processed by two recombinant phytases Phy-A and Phy-C, classified respectively as acid and alkaline phytases, with similar rates of phosphate release compared to their natural substrate.
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hal-03259389 , version 1 (22-03-2023)

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Paternité - Pas d'utilisation commerciale

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Christophe Dussouy, Eric Dubreucq, Patrick Chemardin, Véronique Perrier, Josiane Abadie, et al.. A dansyl-derivatized phytic acid analogue as a fluorescent substrate for phytases: experimental and computational approach. Bioorganic Chemistry, 2021, 110, ⟨10.1016/j.bioorg.2021.104810⟩. ⟨hal-03259389⟩
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