Article Dans Une Revue BMC Bioinformatics Année : 2025

CellMAP: an open-source software tool to batch-process cell topography and stiffness maps collected with an atomic force microscope

Résumé

Atomic force microscopy (AFM) is the gold-standard technique to simultaneously map the morphology and viscoelastic properties of living cells. Although existing software tools, both open-source and from AFM manufacturers, can analyze cells individually, there is a growing need for fast and accessible codes to compile data from multiple cells into a single dataset. To address this, we present CellMAP, a user-friendly software tool that streamlines the batch-processing of AFM-derived topography and stiffness maps of living cells. Our analysis pipeline includes but is not limited to: flattening of the underlying substrate surface, filtering of outlier values, measurement of the cell surface and volume, and measurement of height and stiffness distributions. CellMAP can also generate a composite cell that reflects the height and stiffness properties of an entire cell population.
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hal-04939057 , version 1 (10-02-2025)

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Antoine Allard, Maxime Liboz, Raphaël Crépin, S. Labdi, Olek Maciejak, et al.. CellMAP: an open-source software tool to batch-process cell topography and stiffness maps collected with an atomic force microscope. BMC Bioinformatics, 2025, 26 (1), pp.38. ⟨10.1186/s12859-025-06060-0⟩. ⟨hal-04939057⟩
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