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Article Dans Une Revue Bioanalysis Année : 2012

Choline-containing phospholipids in microdissected human Alzheimer's disease brain senile plaque versus neuropil.

Mathieu Gaudin
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Maï Panchal
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Nicolas Auzeil
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Charles Duyckaerts
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David Touboul

Résumé

Background: Lipidomic studies related to Alzheimer's disease have been reported on either biological fluids or large human brain samples. For a better understanding of the role of lipids, especially during the amyloid-β peptide aggregation, it is crucial to determine the composition of the senile plaque versus the surrounding tissue, that is, the neuropil. Results: A laser microdissection step was added to the analysis by UPLC-MS/MS. Despite the very low amount of sample, two phosphatidylcholines that were significantly depleted in the senile plaque were identified. Conclusion: Changes in the phospholipid content have been shown between senile plaque versus neuropil. Nano HPLC, allowing a complete lipidomic profile, should further improve the results.

Domaines

Chimie organique
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Dates et versions

hal-00742423 , version 1 (16-10-2012)

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Mathieu Gaudin, Maï Panchal, Nicolas Auzeil, Charles Duyckaerts, Alain Brunelle, et al.. Choline-containing phospholipids in microdissected human Alzheimer's disease brain senile plaque versus neuropil.. Bioanalysis, 2012, 4 (17), pp.2153-5159. ⟨10.4155/bio.12.189⟩. ⟨hal-00742423⟩
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