Time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging reveals cholesterol overload in the cerebral cortex of Alzheimer disease patients. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Acta Neuropathologica Année : 2013

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging reveals cholesterol overload in the cerebral cortex of Alzheimer disease patients.

Adina N Lazar
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Claudia Bich
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Maï Panchal
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Nicolas Desbenoit
Vanessa W Petit
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David Touboul
Catherine Marquer
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Charles Duyckaerts
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Résumé

Although cholesterol has been involved in the pathophysiology of Alzheimer disease (AD), its distribution in the cerebral cortex over the course of AD is unknown. We describe an original method to quantify cholesterol distribution using time-of-flight secondary ion mass spectrometry imaging. Cholesterol was unevenly distributed along the cortical thickness, being more abundant close to the white matter, in both control and AD cases. However, the mean cholesterol signal was significantly higher in the lower half of the cortex in AD samples compared to controls. This increase, when converted into cortical layers, was statistically significant for layers III and IV and did not reach significance in layers V + VI, the variability being too high at the interface between grey and white matter. The density of neurofibrillary tangles and of senile plaques was not statistically linked to the abundance of cholesterol. Cholesterol overload thus appears a new and independent alteration of AD cerebral cortex. The structure in which cholesterol accumulates and the mechanism of this accumulation remain to be elucidated.

Domaines

Chimie organique

Dates et versions

hal-00772814 , version 1 (11-01-2013)

Identifiants

Citer

Adina N Lazar, Claudia Bich, Maï Panchal, Nicolas Desbenoit, Vanessa W Petit, et al.. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging reveals cholesterol overload in the cerebral cortex of Alzheimer disease patients.. Acta Neuropathologica, 2013, 125 (1), pp.133-44. ⟨10.1007/s00401-012-1041-1⟩. ⟨hal-00772814⟩
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