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Article Dans Une Revue Science Année : 2019

Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer

Résumé

Drug-resistance dissemination by horizontal gene transfer remains poorly understood at the cellular scale. Using live-cell microscopy, we reveal the dynamics of resistance acquisition by transfer of the Escherichia coli fertility factor–conjugation plasmid encoding the tetracycline-efflux pump TetA. The entry of the single-stranded DNA plasmid into the recipient cell is rapidly followed by complementary-strand synthesis, plasmid-gene expression, and production of TetA. In the presence of translation-inhibiting antibiotics, resistance acquisition depends on the AcrAB-TolC multidrug efflux pump, because it reduces tetracycline concentrations in the cell. Protein synthesis can thus persist and TetA expression can be initiated immediately after plasmid acquisition. AcrAB-TolC efflux activity can also preserve resistance acquisition by plasmid transfer in the presence of antibiotics with other modes of action
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Dates et versions

hal-02162804 , version 1 (22-06-2019)

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Sophie Nolivos, Julien Cayron, Annick A. Dedieu, Adeline Page, Frédéric Delolme, et al.. Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer. Science, 2019, 364 (6442), pp.778-782. ⟨10.1126/science.aav6390⟩. ⟨hal-02162804⟩
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