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Article Dans Une Revue ACS Omega Année : 2019

Advanced Fluorescent Polymer Probes for the Site-Specific Labeling of Proteins in Live Cells Using the HaloTag Technology

Thomas Berki
  • Fonction : Auteur
Anush Bakunts
  • Fonction : Auteur
Damien Duret
  • Fonction : Auteur
Laura Fabre
  • Fonction : Auteur
Andrea Orsi
  • Fonction : Auteur
Eelco van Anken
  • Fonction : Auteur

Résumé

We report the site-specific and covalent bioconjugation of fluorescent polymer chains to proteins in live cells using the HaloTag technology. Polymer chains bearing a Halo-ligand precisely located at their α-chain-end were synthesized in a controlled manner owing to the RAFT polymerization process. They were labeled in lateral position by several organic fluorophores such as AlexaFluor 647. The resulting Haloligand polymer probe was finally shown to selectively recognize and label HaloTag proteins present at the membrane of live cells using confocal fluorescence microscopy. Such a polymer bioconjugation approach holds great promises for various applications ranging from cell imaging to cell surface functionalization.

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hal-02285388 , version 1 (01-12-2020)

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Thomas Berki, Anush Bakunts, Damien Duret, Laura Fabre, Catherine Ladavière, et al.. Advanced Fluorescent Polymer Probes for the Site-Specific Labeling of Proteins in Live Cells Using the HaloTag Technology. ACS Omega, 2019, 4 (7), pp.12841-12847. ⟨10.1021/acsomega.9b01643⟩. ⟨hal-02285388⟩
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