Local light-activation of the Src oncoprotein in an epithelial monolayer promotes collective extrusion - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Communications Physics Année : 2019

Local light-activation of the Src oncoprotein in an epithelial monolayer promotes collective extrusion

Résumé

Transformed isolated cells are usually extruded from normal epithelia and subsequently eliminated. However, multicellular tumors outcompete healthy cells, highlighting the importance of collective effects. Here, we investigate this situation in vitro by controlling in space and time the activity of the Src oncoprotein within a normal Madin-Darby Canine Kidney (MDCK) epithelial cell monolayer. Using an optogenetics approach with cells expressing a synthetic light-sensitive version of Src (optoSrc), we reversibly trigger the oncogenic activity by exposing monolayers to well-defined light patterns. We show that small populations of activated optoSrc cells embedded in the non-transformed monolayer collectively extrude as a tridimensional aggregate and remain alive, while the surrounding normal cells migrate towards the exposed area. This phenomenon requires an interface between normal and transformed cells and is partially reversible. Traction forces show that Src-activated cells either actively extrude or are pushed out by the surrounding cells in a non-autonomous way.
Fichier principal
Vignette du fichier
moitrier bonnet optosrc comm phys 19.pdf (3.55 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02325048 , version 1 (22-10-2019)

Identifiants

Citer

Sarah Moitrier, Nastassia Pricoupenko, Adèle Kerjouan, Christiane I. Oddou, Olivier Destaing, et al.. Local light-activation of the Src oncoprotein in an epithelial monolayer promotes collective extrusion. Communications Physics, 2019, 2 (1), ⟨10.1038/s42005-019-0198-5⟩. ⟨hal-02325048⟩
70 Consultations
28 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More