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Article Dans Une Revue BioImpacts Année : 2019

In vitro and in vivo evaluation of a dextran-graft-polybutylmethacrylate copolymer coated on CoCr metallic stent

Cécilia Delattre
  • Fonction : Auteur
Diego Velasquez
  • Fonction : Auteur
Graciela Pavon-Djavid
  • Fonction : Auteur
Véronique Ollivier
  • Fonction : Auteur
Anna Lokajczyk
  • Fonction : Auteur
Thierry Avramoglou
  • Fonction : Auteur
Virginie Gueguen
  • Fonction : Auteur
Liliane Louedec
  • Fonction : Auteur
Giuseppina Caligiuri
  • Fonction : Auteur
Martine Jandrot-Perrus
  • Fonction : Auteur
Catherine Boisson-Vidal
Didier Letourneur
Anne Meddahi-Pelle
  • Fonction : Auteur

Résumé

Introduction: The major complications of stent implantation are restenosis and late stent thrombosis. PBMA polymers are used for stent coating because of their mechanical properties. We previously synthesized and characterized Dextrangraft-polybutylmethacrylate copolymer (Dex-PBMA) as a potential stent coating. In this study, we evaluated the haemocompatibility and biocompatibility properties of Dex-PBMA in vitro and in vivo. Methods: Here, we investigated: (1) the effectiveness of polymer coating under physiological conditions and its ability to release Tacrolimus®, (2) the capacity of Dex-PBMA to inhibit Staphylococcus aureus adhesion, (3) the thrombin generation and the human platelet adhesion in static and dynamic conditions, (4) the biocompatibility properties in vitro on human endothelial colony forming cells (ECFC) and on mesenchymal stem cells (MSC) and in vivo in rat models, and (5) we implanted Dex-PBMA and Dex-PBMA TAC coated stents in neointimal hyperplasia restenosis rabbit model. Results: Dex-PBMA coating efficiently prevented bacterial adhesion and release Tacrolimus®. Dex-PBMA exhibit haemocompatibility properties under flow and ECFC and MSC compatibility. In vivo, no pathological foreign body reaction was observed neither after intramuscular nor intravascular aortic implantation. After Dex-PBMA and Dex-PBMATAC coated stents 30 days implantation in a restenosis rabbit model, an endothelial cell coverage was observed and the lumen patency was preserved. Conclusion: Based on our findings, Dex-PBMA exhibited vascular compatibility and can potentially be used as a coating for metallic coronary stents.
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Dates et versions

hal-02326072 , version 1 (22-10-2019)

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Paternité - Pas d'utilisation commerciale

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Cécilia Delattre, Diego Velasquez, Caroline Roques, Graciela Pavon-Djavid, Véronique Ollivier, et al.. In vitro and in vivo evaluation of a dextran-graft-polybutylmethacrylate copolymer coated on CoCr metallic stent. BioImpacts, 2019, 9 (1), pp.25-36. ⟨10.15171/bi.2019.04⟩. ⟨hal-02326072⟩
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