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Article Dans Une Revue PLoS Pathogens Année : 2019

Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages

Vincent Le Moigne
Jean-Louis Herrmann

Résumé

Free-living amoebae are thought to represent an environmental niche in which amoeba-resistant bacteria may evolve towards pathogenicity. To get more insights into factors playing a role for adaptation to intracellular life, we characterized the transcriptomic activities of the emerging pathogen Mycobacterium abscessus in amoeba and murine macrophages (Mϕ) and compared them with the intra-amoebal transcriptome of the closely related, but less pathogenic Mycobacterium chelonae. Data on up-regulated genes in amoeba point to proteins that allow M. abscessus to resist environmental stress and induce defense mechanisms , as well as showing a switch from carbohydrate carbon sources to fatty acid metabolism. For eleven of the most upregulated genes in amoeba and/or Mϕ, we generated individual gene knockout M. abscessus mutant strains, from which ten were found to be attenuated in amoeba and/or Mϕ in subsequence virulence analyses. Moreover, transfer of two of these genes into the genome of M. chelonae increased the intra-Mϕ survival of the recombinant strain. One knockout mutant that had the gene encoding Eis N-acetyl transfer-ase protein (MAB_4532c) deleted, was particularly strongly attenuated in Mϕ. Taken together, M. abscessus intra-amoeba and intra-Mϕ transcriptomes revealed the capacity of M. abscessus to adapt to an intracellular lifestyle, with amoeba largely contributing to the enhancement of M. abscessus intra-Mϕ survival.
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hal-02379161 , version 1 (27-01-2020)

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Violaine Dubois, Alexandre Pawlik, Anouchka Bories, Vincent Le Moigne, Odile Sismeiro, et al.. Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages. PLoS Pathogens, 2019, 15 (11), pp.e1008069. ⟨10.1371/journal.ppat.1008069⟩. ⟨hal-02379161⟩
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