The small RNA diversity from Medicago truncatula roots under biotic interactions evidences the environmental plasticity of the miRNAome. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Genome Biology Année : 2014

The small RNA diversity from Medicago truncatula roots under biotic interactions evidences the environmental plasticity of the miRNAome.

Christine Lelandais-Brière
  • Fonction : Auteur
  • PersonId : 1355839
  • IdRef : 073427055
Florian Frugier
Jean-Philippe Combier

Résumé

BackgroundLegume roots show a remarkable plasticity to adapt their architecture to biotic and abiotic constraints, including symbiotic interactions. However, global analysis of miRNA regulation in roots is limited, and a global view of the evolution of miRNA-mediated diversification in different ecotypes is lacking.ResultsIn the model legume Medicago truncatula, we analyze the small RNA transcriptome of roots submitted to symbiotic and pathogenic interactions. Genome mapping and a computational pipeline identify 416 miRNA candidates, including known and novel variants of 78 miRNA families present in miRBase. Stringent criteria of pre-miRNA prediction yield 52 new mtr-miRNAs, including 27 miRtrons. Analyzing miRNA precursor polymorphisms in 26 M. truncatula ecotypes identifies higher sequence polymorphism in conserved rather than Medicago-specific miRNA precursors. An average of 19 targets, mainly involved in environmental responses and signalling, is predicted per novel miRNA. We identify miRNAs responsive to bacterial and fungal pathogens or symbionts as well as their related Nod and Myc-LCO symbiotic signals. Network analyses reveal modules of new and conserved co-expressed miRNAs that regulate distinct sets of targets, highlighting potential miRNA-regulated biological pathways relevant to pathogenic and symbiotic interactions.ConclusionsWe identify 52 novel genuine miRNAs and large plasticity of the root miRNAome in response to the environment, and also in response to purified Myc/Nod signaling molecules. The new miRNAs identified and their sequence variation across M. truncatula ecotypes may be crucial to understand the adaptation of root growth to the soil environment, notably in the agriculturally important legume crops.
Fichier principal
Vignette du fichier
2014_Formey_Genome Biology_1.pdf (2.71 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02635888 , version 1 (27-05-2020)

Licence

Paternité

Identifiants

Citer

Damien Formey, Erika Sallet, Christine Lelandais-Brière, Cécile Ben, Pilar Bustos-Sanmamed, et al.. The small RNA diversity from Medicago truncatula roots under biotic interactions evidences the environmental plasticity of the miRNAome.. Genome Biology, 2014, 15 (9), pp.457. ⟨10.1186/s13059-014-0457-4⟩. ⟨hal-02635888⟩
34 Consultations
46 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More