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Article Dans Une Revue Ecotoxicology and Environmental Safety Année : 2011

Genotoxic damages in zebrafish submitted to a polymetallic gradient displayed by the Lot River (France)

Résumé

Genotoxic effects of a polymetallic pollution gradient displayed by the Lot River and one of its tributary have been assessed on zebrafish Danio rerio. Three methods were compared: RAPD-PCR, the comet assay, and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) formation. The fishes were exposed for 14 days to waters collected from three stations: Joanis, a site polluted by cadmium (Cd) and zinc (Zn) (mean concentrations: 15. γg Cd/L and 550. γg Zn/L), Bouillac (mean concentrations: 0.55. γg Cd/L and 80. γg Zn/L), and Boisse-Penchot, a reference station (mean concentrations:<0.05. γg Cd/L and 7. γg Zn/L). The quantitative RAPD-PCR methodology proved to be sensitive enough to unmask metal genotoxicity after 3 and 7 days of exposure to Joanis water and after 14 days to Bouillac water, whereas the comet assay only detected DNA damages at the most contaminated station (Joanis). The 8-oxodG quantification was not sensitive enough to be used in zebrafish under these environmental conditions. © 2011 Elsevier Inc.

Dates et versions

hal-02954959 , version 1 (01-10-2020)

Identifiants

Citer

N. Orieux, S. Cambier, P. Gonzalez, B. Morin, C. Adam, et al.. Genotoxic damages in zebrafish submitted to a polymetallic gradient displayed by the Lot River (France). Ecotoxicology and Environmental Safety, 2011, 74 (4), pp.974-983. ⟨10.1016/j.ecoenv.2011.01.008⟩. ⟨hal-02954959⟩
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