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Pré-Publication, Document De Travail Année : 2020

A Phelipanche ramosa KAI2 Protein Perceives enzymatically Strigolactones and Isothiocyanates

Alexandre de Saint Germain
Anse Jacobs
  • Fonction : Auteur
Guillaume Brun
  • Fonction : Auteur
Jean-Bernard Pouvreau
Lukas Braem
  • Fonction : Auteur
David Cornu
Guillaume Clavé
Emmanuelle Baudu
  • Fonction : Auteur
Vincent Steinmetz
  • Fonction : Auteur
Vincent Servajean
  • Fonction : Auteur
Susann Wicke
  • Fonction : Auteur
Kris Gevaert
Philippe Simier
Sofie Goormachtig
  • Fonction : Auteur
Philippe Delavault

Résumé

Phelipanche ramosa is an obligate root-parasitic weed threatening major crops in central Europe. For its germination, it has to perceive various structurally diverging host-exuded signals, including isothiocyanates (ITCs) and strigolactones (SLs). However, the receptors involved are still uncharacterized. Here, we identified five putative SL receptors in P. ramosa , of which PrKAI2d3 is involved in seed germination stimulation. We established the high plasticity of PrKAI2d3, allowing interaction with different chemicals, including ITCs. The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants. We provide evidence that the PrKAI2d3 enzymatic activity confers hypersensitivity to SLs. Additionally, we demonstrated that methylbutenolide-OH binds PrKAI2d3 and stimulates P. ramosa germination with a bioactivity comparable to that of ITCs. This study highlights that P. ramosa has extended its signal perception system during evolution, a fact to be considered in the development of specific and efficient biocontrol methods.
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Dates et versions

hal-02997223 , version 1 (10-11-2020)

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Alexandre de Saint Germain, Anse Jacobs, Guillaume Brun, Jean-Bernard Pouvreau, Lukas Braem, et al.. A Phelipanche ramosa KAI2 Protein Perceives enzymatically Strigolactones and Isothiocyanates. 2020. ⟨hal-02997223⟩
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