CRISPR-mediated biocontainment - CNRS - Centre national de la recherche scientifique Access content directly
Preprints, Working Papers, ... Year : 2020

CRISPR-mediated biocontainment

Cory J Smith
  • Function : Author
Parastoo Khoshakhlagh
  • Function : Author
Raphael Ferreira
  • Function : Author
Marc Güell
  • Function : Author
Khaled Said
  • Function : Author
Ramazan Yildiz
  • Function : Author
Matthew Dysart
  • Function : Author
Stan Wang
  • Function : Author
David Thompson
  • Function : Author
George M Church
  • Function : Author
  • PersonId : 1030371


We have exploited the repetitive nature of transposable elements of the human genome to generate synthetic circuits. Transposable elements such as LINE-1 and Alu have successfully replicated in mammalian genomes throughout evolution to reach a copy number ranging from thousands to more than a million. Targeting these repetitive elements with programmable DNA nucleases such as CRISPR-Cas9 rapidly induce extremely high levels of cell death. We use this genotoxic feature to build synthetic biocontainment circuits: CRISPR defense system (CRISPR-DS) capable of preventing CRISPR genome editing, and we introduce the proof-of-concept of CRISPR Safety-Switch, an inducible, stringent and non-leaky kill-switch capable of clearing out cell lines resistant to DNA breaks. .
Fichier principal
Vignette du fichier
2020.02.03.922146v1.full.pdf (1.46 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03027524 , version 1 (27-11-2020)



Oscar Castanon, Cory J Smith, Parastoo Khoshakhlagh, Raphael Ferreira, Marc Güell, et al.. CRISPR-mediated biocontainment. 2020. ⟨hal-03027524⟩
30 View
57 Download



Gmail Facebook X LinkedIn More