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Journal Articles International Journal of Molecular Sciences Year : 2019

Toxicological Evaluation of SiO2 Nanoparticles by Zebrafish Embryo Toxicity Test

Sandra Vranic
  • Function : Author
Yasuhito Shimada
  • Function : Author
Sahoko Ichihara
  • Function : Author
Masayuki Kimata
  • Function : Author
Wenting Wu
  • Function : Author
Toshio Tanaka
  • Function : Author
Lang Tran
  • Function : Author
Gaku Ichihara
  • Function : Author


As the use of nanoparticles (NPs) is increasing, the potential toxicity and behavior of NPs in living systems need to be better understood. Our goal was to evaluate the developmental toxicity and bio-distribution of two different sizes of fluorescently-labeled SiO2 NPs, 25 and 115 nm, with neutral surface charge or with different surface functionalization, rendering them positively or negatively charged, in order to predict the effect of NPs in humans. We performed a zebrafish embryo toxicity test (ZFET) by exposing the embryos to SiO2 NPs starting from six hours post fertilization (hpf). Survival rate, hatching time, and gross morphological changes were assessed at 12, 24, 36, 48, 60, and 72 hpf. We evaluated the effect of NPs on angiogenesis by counting the number of sub-intestinal vessels between the second and seventh intersegmental vessels and gene expression analysis of vascular endothelial growth factor (VEGF) and VEGF receptors at 72 hpf. SiO2 NPs did not show any adverse effects on survival rate, hatching time, gross morphology, or physiological angiogenesis. We found that SiO2 NPs were trapped by the chorion up until to the hatching stage. After chemical removal of the chorion (dechorionation), positively surface-charged SiO2 NPs (25 nm) significantly reduced the survival rate of the fish compared to the control group. These results indicate that zebrafish chorion acts as a physical barrier against SiO2 NPs, and removing the chorions in ZFET might be necessary for evaluation of toxicity of NPs.
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Dates and versions

hal-03201976 , version 1 (16-11-2022)



Sandra Vranic, Yasuhito Shimada, Sahoko Ichihara, Masayuki Kimata, Wenting Wu, et al.. Toxicological Evaluation of SiO2 Nanoparticles by Zebrafish Embryo Toxicity Test. International Journal of Molecular Sciences, 2019, 20 (4), pp.882. ⟨10.3390/ijms20040882⟩. ⟨hal-03201976⟩
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