Isolation of Primary Hepatocytes for Testing Tumor Penetrating Peptides - Archive ouverte HAL Access content directly
Journal Articles Methods in molecular biology (Clifton, N.J.) Year : 2022

Isolation of Primary Hepatocytes for Testing Tumor Penetrating Peptides

Abstract

Methods for isolation of human primary cells is an important tool to be able to test the effect of several drugs for the treatment of diseases. Protocols have been described for the isolation of healthy and tumoral hepatocytes; however, the quality and the amount of the isolated cells is not satisfactory. We describe here protocols for the isolation of healthy and tumoral hepatocytes as well as the use of tumor penetrating and interfering peptides as new therapeutics. Peptides are showed to be safe drugs and taking into account recent improvements in peptide administration, biodelivery, and bioavailability. Tumor penetrating peptides fused to an interfering peptide have a great potential to become therapeutic agents.
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hal-03677209 , version 1 (24-05-2022)

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Eric Savier, Pierre Tuffery, Heriberto Bruzzoni-Giovanelli, Angelita Rebollo. Isolation of Primary Hepatocytes for Testing Tumor Penetrating Peptides. Methods in molecular biology (Clifton, N.J.), 2022, 2383, pp.413-427. ⟨10.1007/978-1-0716-1752-6_26⟩. ⟨hal-03677209⟩
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