The $Toxoplasma$ effector GRA28 promotes parasite dissemination by inducing dendritic cell-like migratory properties in infected macrophages - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Cell Host & Microbe Année : 2022

The $Toxoplasma$ effector GRA28 promotes parasite dissemination by inducing dendritic cell-like migratory properties in infected macrophages

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Upon pathogen detection, macrophages normally stay sessile in tissues while dendritic cells (DCs) migrate to secondary lymphoid tissues. The obligate intracellular protozoan Toxoplasma gondii exploits the trafficking of mononuclear phagocytes for dissemination via unclear mechanisms. We report that, upon T. gondii infection, macrophages initiate the expression of transcription factors normally attributed to DCs, upregulate CCR7 expression with a chemotactic response, and perform systemic migration when adoptively transferred into mice. We show that parasite effector GRA28, released by the MYR1 secretory pathway, cooperates with host chromatin remodelers in the host cell nucleus to drive the chemotactic migration of parasitized macrophages. During in vivo challenge studies, bone marrow-derived macrophages infected with wild-type T. gondii outcompeted those challenged with MYR1- or GRA28-deficient strains in migrating and reaching secondary organs. This work reveals how an intracellular parasite hijacks chemotaxis in phagocytes and highlights a remarkable migratory plasticity in differentiated cells of the mononuclear phagocyte system.
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hal-03862330 , version 1 (21-11-2022)

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Arne L. ten Hoeve, Laurence Braun, Matias E Rodriguez, Gabriela C Olivera, Alexandre Bougdour, et al.. The $Toxoplasma$ effector GRA28 promotes parasite dissemination by inducing dendritic cell-like migratory properties in infected macrophages. Cell Host & Microbe, 2022, 30, pp.1570 - 1588. ⟨10.1016/j.chom.2022.10.001⟩. ⟨hal-03862330⟩
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