β-Catenin activation and illicit receptor expression in adrenocortical cells - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Endocrine-Related Cancer Année : 2022

β-Catenin activation and illicit receptor expression in adrenocortical cells

Julie Le Mestre
  • Fonction : Auteur
Michaël Thomas
  • Fonction : Auteur
Céline Duparc
Pierre Val
Clémence Bures
  • Fonction : Auteur
Jean-Jacques Tuech
  • Fonction : Auteur
Jean-Christophe Sabourin
  • Fonction : Auteur
Stéphanie Baert-Desurmont
  • Fonction : Auteur
Hervé Lefebvre
Estelle Louiset
  • Fonction : Auteur

Résumé

Adrenal cortisol-producing tumors can express illicit membrane receptors such as luteinizing hormone (LH), glucose-dependent insulinotropic peptide (GIP) or type 4 and 7 serotonin (5-HT 4/7 ) receptors. Abnormal expression of the LH receptor (LH-R) has been ascribed to the activation of the Wnt/β-catenin signaling pathway in adrenocortical cells. In the present study, we have investigated whether β-catenin activation may also trigger the illegitimate expression of GIP and 5-HT receptors. Three models of β-catenin activation in adrenocortical cells were used: an APC -mutated adrenocortical tumor, human-transfected adrenocortical cells and genetically modified mouse adrenal glands. The methods employed include quantitative reverse transcription PCR, immunohistochemistry and measurement of cortisol secretion by cultured tumor cells. Abnormal expression of the GIP, 5-HT 7 and LH receptors was observed in the APC -mutated adrenocortical tumor tissue. In addition, GIP, 5-HT and human chorionic gonadotropin stimulated cortisol production from tumor cells in primary culture. Conversely, only the LHCGR was upregulated in human and mouse adrenocortical cells harboring the activation of β-catenin. Moreover, LH-R immunoreactivity was detected in clusters of zona fasciculata cells in the β-catenin-activated mouse model. Our data indicate that activation of the β-catenin signaling pathway can promote the illicit expression of functional LH-Rs in adrenal zona fasciculata cells but does not favor the abnormal expression of GIP and 5-HT receptors.
Fichier non déposé

Dates et versions

hal-03864819 , version 1 (22-11-2022)

Identifiants

Citer

Julie Le Mestre, Michaël Thomas, Céline Duparc, Pierre Val, Clémence Bures, et al.. β-Catenin activation and illicit receptor expression in adrenocortical cells. Endocrine-Related Cancer, 2022, 29 (3), pp.151-162. ⟨10.1530/ERC-21-0298⟩. ⟨hal-03864819⟩
15 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More