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Article Dans Une Revue Current Issues in Molecular Biology Année : 2023

A Comparative Analysis of NOX4 Protein Expression in Malignant and Non-Malignant Thyroid Tumors

Salma Fenniche
  • Fonction : Auteur
Mohamed Oukabli
  • Fonction : Auteur
Yassire Oubaddou
Hafsa Chahdi
  • Fonction : Auteur
Amal Damiri
  • Fonction : Auteur
Abir Alghuzlan
Abdelilah Laraqui
  • Fonction : Auteur
Nadia Dakka
  • Fonction : Auteur
Youssef Bakri
  • Fonction : Auteur
Rabii Ameziane El Hassani

Résumé

The comparative analysis of the expression of the reactive oxygen species-generating NADPH oxidase NOX4 from TCGA data shows that the NOX4 transcript is upregulated in papillary thyroid carcinomas (PTC)-BRAF V600E tumors compared to PTC-BRAF wt tumors. However, a comparative analysis of NOX4 at the protein level in malignant and non-malignant tumors is missing. We explored NOX4 protein expression by immunohistochemistry staining in malignant tumors (28 classical forms of PTC (C-PTC), 17 follicular variants of PTC (F-PTC), and three anaplastic thyroid carcinomas (ATCs)) and in non-malignant tumors (six lymphocytic thyroiditis, four Graves' disease, ten goiters, and 20 hyperplasias). We detected the BRAF V600E mutation by Sanger sequencing and digital droplet PCR. The results show that NOX4 was found to be higher (score ≥ 2) in C-PTC (92.9%) compared to F-PTC (52.9%) and ATC (33.3%) concerning malignant tumors. Interestingly, all C-PTC-BRAF V600E expressed a high score for NOX4 at the protein level, strengthening the positive correlation between the BRAF V600E mutation and NOX4 expression. In addition, independent of the mutational status of BRAF, we observed that 90% of C-PTC infiltrating tumors showed high NOX4 expression, suggesting that NOX4 may be considered a complementary biomarker in PTC aggressiveness. Interestingly, NOX4 was highly expressed in non-malignant thyroid diseases with different subcellular localizations.
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Dates et versions

hal-04259403 , version 1 (26-10-2023)

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Citer

Salma Fenniche, Mohamed Oukabli, Yassire Oubaddou, Hafsa Chahdi, Amal Damiri, et al.. A Comparative Analysis of NOX4 Protein Expression in Malignant and Non-Malignant Thyroid Tumors. Current Issues in Molecular Biology, 2023, 45 (7), pp.5811 - 5823. ⟨10.3390/cimb45070367⟩. ⟨hal-04259403⟩
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