Pseudomonas aeruginosa LecB suppresses immune responses by inhibiting transendothelial migration - CNRS - Centre national de la recherche scientifique Access content directly
Journal Articles EMBO Reports Year : 2023

Pseudomonas aeruginosa LecB suppresses immune responses by inhibiting transendothelial migration

Winfried Römer

Abstract

Abstract Pseudomonas aeruginosa is a Gram‐negative bacterium causing morbidity and mortality in immuno‐compromised humans. It produces a lectin, LecB, that is considered a major virulence factor, however, its impact on the immune system remains incompletely understood. Here we show that LecB binds to endothelial cells in human skin and mice and disrupts the transendothelial passage of leukocytes in vitro . It impairs the migration of dendritic cells into the paracortex of lymph nodes leading to a reduced antigen‐specific T cell response. Under the effect of the lectin, endothelial cells undergo profound cellular changes resulting in endocytosis and degradation of the junctional protein VE‐cadherin, formation of an actin rim, and arrested cell motility. This likely negatively impacts the capacity of endothelial cells to respond to extracellular stimuli and to generate the intercellular gaps for allowing leukocyte diapedesis. A LecB inhibitor can restore dendritic cell migration and T cell activation, underlining the importance of LecB antagonism to reactivate the immune response against P. aeruginosa infection.
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Origin Publication funded by an institution

Dates and versions

hal-04261591 , version 1 (15-11-2023)

Identifiers

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Janina Sponsel, Yubing Guo, Lutfir Hamzam, Alice Lavanant, Annia Pérez-Riverón, et al.. Pseudomonas aeruginosa LecB suppresses immune responses by inhibiting transendothelial migration. EMBO Reports, 2023, 24 (4), pp.e55971. ⟨10.15252/embr.202255971⟩. ⟨hal-04261591⟩
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