Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging - CNRS - Centre national de la recherche scientifique
Article Dans Une Revue STAR Protocols Année : 2023

Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging

Protocole pour exprimer localement cxcl12a pendant le développement de l'organe olfactif du poisson zèbre en combinant IR-LEGO et l'imagerie en direct

Résumé

Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ development 1 using a combination of IR-LEGO and live imaging. We describe steps for implementing IR-LEGO, biological sample preparation, live imaging, data collection, and analysis. This protocol can be applied to virtually any genetically modified experimental organism.
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Dates et versions

hal-04293547 , version 1 (18-11-2023)

Identifiants

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Marie Zilliox, Vanessa Tillement, Thomas Mangeat, Sophie Polès, Patrick Blader, et al.. Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live imaging. STAR Protocols, 2023, 4 (3), pp.102538. ⟨10.1016/j.xpro.2023.102538⟩. ⟨hal-04293547⟩
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