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Pré-Publication, Document De Travail (Preprint/Prepublication) Année : 2023

Demixing fluorescence time traces transmitted by multimode fibers

Claudio Moretti
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  • PersonId : 1313360
Fernando Soldevila
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Enora Brémont
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Cathie Ventalon
Sylvain Gigan
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  • PersonId : 966044

Résumé

Fiber photometry is a significantly less invasive method compared to other deep brain imaging microendoscopy approaches due to the use of thin multimode fibers (MMF diameter < 500 µm). Nevertheless, the transmitted signals get scrambled upon propagation within the MMF, thus limiting the technique's potential in resolving temporal readouts with cellular resolution. Here, we demonstrate how to separate the time trace signals of several fluorescent sources probed by a thin (≈ 200 µm) MMF with typical implantable length in a mouse brain. We disentangled several spatio-temporal fluorescence signals by using a general unconstrained non-negative matrix factorization (NMF) algorithm directly on the raw video data. Furthermore, we show that commercial and low-cost open-source miniscopes display enough sensitivity to image the same fluorescence patterns seen in our proof of principle experiment, suggesting that a whole new avenue for novel minimally invasive deep brain studies with multimode fibers in freely-behaving mice is possible.
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Dates et versions

hal-04299871 , version 1 (22-11-2023)

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Citer

Caio Vaz Rimoli, Claudio Moretti, Fernando Soldevila, Enora Brémont, Cathie Ventalon, et al.. Demixing fluorescence time traces transmitted by multimode fibers. 2023. ⟨hal-04299871⟩
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