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Pré-Publication, Document De Travail Année : 2023

MicroAnnot: A Dedicated Workflow for Accurate Microsporidian Genome Annotation

Jérémy Tournayre
Valérie Polonais
Ivan Wawrzyniak
Reginald Florian Akossi
  • Fonction : Auteur
Nicolas Parisot
  • Fonction : Auteur
Fréréric Delbac
Pierre Souvignet
Matthieu Reichstadt
  • Fonction : Auteur
Eric Peyretaillade
  • Fonction : Auteur

Résumé

With nearly 1,700 species, Microsporidia represent a group of obligate intracellular eukaryotes with veterinary, economic and medical impacts. To help in understanding the biological functions of these microorganisms, complete genome sequencing is used routinely. Nevertheless, the proper prediction of their gene catalogue is challenging due to taxon-specific evolutionary features. As innovative genome annotation strategies are needed to obtain a representative snapshot of the overall lifestyle of these parasites, the MicroAnnot tool, a dedicated workflow for microsporidian sequence annotation using data from curated databases of accurately annotated microsporidian genes, has been developed. Furthermore, specific modules have been implemented to perform small genes (< 300bp) and transposable element identification. Finally, functional annotation was performed using the signature-based InterProScan software. MicroAnnot’s accuracy has been verified by the re-annotation of four microsporidian genomes for which structural annotation had previously been validated. With its comparative approach and transcriptional signal identification method, MicroAnnot provides accurate prediction of translation initiation sites, efficient identification of transposable elements, as well as high specificity and sensitivity for microsporidian genes including those under 300 bp. Thanks to its web interface (https://microannot.org), MicroAnnot is available to the community to ensure high quality annotation of microsporidian genomes.

Dates et versions

hal-04304127 , version 1 (24-11-2023)

Identifiants

Citer

Jérémy Tournayre, Valérie Polonais, Ivan Wawrzyniak, Reginald Florian Akossi, Nicolas Parisot, et al.. MicroAnnot: A Dedicated Workflow for Accurate Microsporidian Genome Annotation. 2023. ⟨hal-04304127⟩
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