Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics - CNRS - Centre national de la recherche scientifique
Article Dans Une Revue Science Advances Année : 2024

Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics

Angeliki Platania
Cathie Erb
Mariano Barbieri
Bastien Molcrette
Erwan Grandgirard
Marit de Kort
  • Fonction : Auteur
Wim Pomp
Karen Meaburn
  • Fonction : Auteur
Tiegh Taylor
  • Fonction : Auteur
Virlana Shchuka
Mariia Nazarova
Guilherme Monteiro Oliveira
Jennifer Mitchell
Evi Soutoglou
  • Fonction : Auteur
Tineke Lenstra
Nacho Molina
  • Fonction : Auteur
Argyris Papantonis
Tom Sexton
Kerstin Bystricky

Résumé

The spatiotemporal configuration of genes with distal regulatory elements is believed to be crucial for transcriptional control, but full mechanistic understanding is lacking. We combine simultaneous live tracking of pairs of genomic loci and nascent transcripts with molecular dynamics simulations to assess the Sox2 gene and its enhancer. We find that both loci exhibit more constrained mobility than control sequences due to stalled cohesin at CCCTC-binding factor sites. Strikingly, enhancer mobility becomes constrained on transcriptional firing, homogenizing its dynamics with the gene promoter, suggestive of their cotranscriptional sharing of a nuclear microenvironment. Furthermore, we find transcription and loop extrusion to be antagonistic processes constraining regulatory loci. These findings indicate that modulating chromatin mobility can be an additional, underestimated means for effective gene regulation.
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Dates et versions

hal-04838464 , version 1 (14-12-2024)

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Citer

Angeliki Platania, Cathie Erb, Mariano Barbieri, Bastien Molcrette, Erwan Grandgirard, et al.. Transcription processes compete with loop extrusion to homogenize promoter and enhancer dynamics. Science Advances , 2024, 10 (50), ⟨10.1126/sciadv.adq0987⟩. ⟨hal-04838464⟩
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