Chemotaxonomic survey of plant GIPCs
Résumé
The plant kingdom offers remarkable phylogenetic and biochemical diversity. Among the major evolutionary lineages of plants, the Angiosperms constitute the most diverse group, encompassing the majority of terrestrial plants. The Talence Botanical Garden collection, comprising 268 Spermatophyte specimens representing 56 orders and 168 families, has been designed to reflect this diversity. This richness is crucial for studying inter-species and inter-order variability, and for identifying correlations between evolutionary lineages and biochemical profiles. Although Glycosyl-Inositol-Phospho-Ceramides (GIPCs) are the main sphingolipids of plant tissues, they remain poorly characterized in term of structures. This lack of information, notably with regard to polar heads, currently hampers the understanding of GIPC functions in biological systems. This situation prompted us to undertake a large scale-analysis of plant GIPCs. A broad study of the GIPC polar heads of 23 plant species from algae to monocots showed that polar head structures are largely unknown and vary widely across different biological taxa (1). We determined a sphingolipidomic profile based on the targeted analysis of glucosylceramides (GlcCer) and Glycosyl-Inositol-Phospho-Ceramides (GIPC) from 58 species of Spermatophytes—including Gymnosperms and Angiosperms covering species from the ANA grade, Magnoliids, Monocots, Ceratophyllales, and Eudicots. This dataset will allow us to measure the GIPC levels for each sample and initially observe whether a trend emerges within between GIPC series A (2 sugars) in dicot and GIPC series B (3 sugars) in monocot, as well as the relative distribution of their decorated forms (-OH, -NH2, and -NAc) for each botanical sample.
