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Article Dans Une Revue Desalination and Water Treatment Année : 2016

RP-HPLC and spectroscopic characterization of Suwannee River water NOM after concentrated urea treatment and dialysis

Résumé

Suwannee River natural organic matter (SRNOM) was treated by 7 M urea and then purified by dialysis on 10 kDa membrane. The untreated SRNOM and treated (USRNOM) samples were examined using UV–visible and fluorescence spectroscopies and reversed-phase high-performance liquid chromatography (RP-HPLC) with online absorbance and fluorescence detection. USRNOM was 1.5-fold more absorbing at 280 nm than SRNOM and four fold less fluorescent than SRNOM upon excitation at 270 nm. RP-HPLC analyses of the two samples revealed that USRNOM was somewhat more hydrophobic than SRNOM and both samples contained at least two groups of HS-like fluorophores with different hydrophobicity and protein-like fluorophore(s). Data indicate that protein-like fluorophores were not lost during dialysis. They showed hydrophobic properties and seemed highly fluorescent. HS-like and protein-like fluorophores from water NOM could be successfully separated by RP-HPLC. This raises the prospect of their further research and identification and could be significant for future NOM chemical structure characterization.
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Dates et versions

hal-01288152 , version 1 (14-03-2016)

Identifiants

Citer

Olga E. Trubetskaya, Claire Richard, Guillaume Voyard, Victor V. Marchenkov, Oleg A. Trubetskoj. RP-HPLC and spectroscopic characterization of Suwannee River water NOM after concentrated urea treatment and dialysis. Desalination and Water Treatment, 2016, 57, pp.5358-5364. ⟨10.1080/19443994.2014.1003610⟩. ⟨hal-01288152⟩
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