Crystal Structure of Leucotoxin S Component - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2004

Crystal Structure of Leucotoxin S Component

Résumé

Staphylococcal leucocidins and γ-hemolysins (leucotoxins) are bi-component toxins that form lytic transmembrane pores. Their cytotoxic activities require the synergistic association of a class S component and a class F component, produced as water-soluble monomers that form hetero-oligomeric membrane-associated complexes. Strains that produce the Panton-Valentine leucocidin are clinically associated with cutaneous lesions and community-acquired pneumonia. In a previous study, we determined the crystal structure of the F monomer from the Panton-Valentine leucocidin. To derive information on the second component of the leucotoxins, the x-ray structure of the S protein from the Panton-Valentine leucocidin was solved to 2.0 Å resolution using a tetragonal crystal form that contains eight molecules in the asymmetric unit. The structure demonstrates the different conformation of the domain involved in membrane contacts and illustrates sequence and tertiary structure variabilities of the pore-forming leucotoxins. Mutagenesis studies at a key surface residue (Thr-28) further support the important role played by these microheterogeneities for the assembly of the bipartite leucotoxins.
Fichier principal
Vignette du fichier
guillet-jbiolchem04.pdf (966.41 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-03004162 , version 1 (13-11-2020)

Identifiants

Citer

Valérie Guillet, Pierre Roblin, Sandra Werner, Manuela Coraiola, Gianfranco Menestrina, et al.. Crystal Structure of Leucotoxin S Component. Journal of Biological Chemistry, 2004, 279, pp.41028 - 41037. ⟨10.1074/jbc.m406904200⟩. ⟨hal-03004162⟩
14 Consultations
61 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More