Doped Graphene To Mimic the Bacterial NADH Oxidase for One-Step NAD + Supplementation in Mammals - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of the American Chemical Society Année : 2023

Doped Graphene To Mimic the Bacterial NADH Oxidase for One-Step NAD + Supplementation in Mammals

Résumé

Nicotinamide adenine dinucleotide (NAD) is a critical regulator of metabolic networks, and declining levels of its oxidized form, NAD+, are closely associated with numerous diseases. While supplementing cells with precursors needed for NAD+ synthesis has shown poor efficacy in combatting NAD+ decline, an alternative strategy is the development of synthetic materials that catalyze the oxidation of NADH into NAD+, thereby taking over the natural role of the NADH oxidase (NOX) present in bacteria. Herein, we discovered that metal-nitrogen-doped graphene (MNGR) materials can catalyze the oxidation of NADH into NAD+. Among MNGR materials with different transition metals, Fe-, Co-, and Cu-NGR displayed strong catalytic activity combined with >80% conversion of NADH into NAD+, similar specificity to NOX for abstracting hydrogen from the pyridine ring of nicotinamide, and higher selectivity than 51 other nanomaterials. The NOX-like activity of FeNGR functioned well in diverse cell lines. As a proof of concept of the in vivo application, we showed that FeNGR could specifically target the liver and remedy the metabolic flux anomaly in obesity mice with NAD+-deficient cells. Overall, our study provides a distinct insight for exploration of drug candidates by design of synthetic materials to mimic the functions of unique enzymes (e.g., NOX) in bacteria.
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Dates et versions

hal-04018519 , version 1 (07-03-2023)

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Xi Liu, Jingkun Li, Andrea Zitolo, Meng Gao, Jun Jiang, et al.. Doped Graphene To Mimic the Bacterial NADH Oxidase for One-Step NAD + Supplementation in Mammals. Journal of the American Chemical Society, 2023, 145 (5), pp.3108-3120. ⟨10.1021/jacs.2c12336⟩. ⟨hal-04018519⟩
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