Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the <i>Paramecium</i> sexual cycle - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 2023

Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle

Résumé

Multinucleate cells are found in many eukaryotes, but how multiple nuclei coordinate their functions is still poorly understood. In the cytoplasm of the ciliate Paramecium tetraurelia , two micronuclei (MIC) serving sexual reproduction coexist with a somatic macronucleus (MAC) dedicated to gene expression. During sexual processes, the MAC is progressively destroyed while still ensuring transcription, and new MACs de v elop from copies of the zygotic MIC. Several gene clusters are successively induced and switched off before vegetative growth resumes. Concomitantly, programmed genome rearrangement (PGR) remo v es transposons and their relics from the ne w MACs. De v elopment of the new MACs is controlled by the old MAC, since the latter expresses genes involved in PGR, including the PGM gene encoding the essential PiggyMac endonuclease that clea v es the ends of eliminated sequences. Using RNA deep sequencing and transcriptome analy sis, w e sho w that impairing PGR upregulates k e y kno wn PGR genes, together with ∼600 other genes possibly also in v olv ed in PGR. Among these genes, 42% are no longer induced when no new MACs are formed, including 180 genes that are co-expressed with PGM under all tested conditions. We propose that bi-directional crosstalk between the two coexisting generations of MACs links gene expression to the progression of MAC development.
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hal-04291944 , version 1 (17-11-2023)

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Mélanie Bazin-Gélis, Evangelia Eleftheriou, Coralie Zangarelli, Gaëlle Lelandais, Linda Sperling, et al.. Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle. Nucleic Acids Research, In press, ⟨10.1093/nar/gkad1006⟩. ⟨hal-04291944⟩
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