Transcription-induced domains form the elementary constraining building blocks of bacterial chromosomes - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Nature Structural and Molecular Biology Année : 2024

Transcription-induced domains form the elementary constraining building blocks of bacterial chromosomes

Résumé

Transcription generates local topological and mechanical constraints on the DNA fiber, leading to the generation of supercoiled chromosome domains in bacteria. However, the global impact of transcription on chromosome organization remains elusive, as the scale of genes and operons in bacteria remains well below the resolution of chromosomal contact maps generated using Hi-C (~5–10 kb). Here we combined sub-kb Hi-C contact maps and chromosome engineering to visualize individual transcriptional units. We show that transcriptional units form discrete three-dimensional transcription-induced domains that impose mechanical and topological constraints on their neighboring sequences at larger scales, modifying their localization and dynamics. These results show that transcriptional domains constitute primary building blocks of bacterial chromosome folding and locally impose structural and dynamic constraints.
Fichier principal
Vignette du fichier
s41594-023-01178-2.pdf (10.14 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

hal-04468362 , version 1 (22-02-2024)

Licence

Paternité

Identifiants

Citer

Amaury Bignaud, Charlotte Cockram, Céline Borde, Justine Groseille, Eric Allemand, et al.. Transcription-induced domains form the elementary constraining building blocks of bacterial chromosomes. Nature Structural and Molecular Biology, 2024, ⟨10.1038/s41594-023-01178-2⟩. ⟨hal-04468362⟩
97 Consultations
2 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More